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Serial Dilution Lab Report Paper4/22/2021
That way, every time you open a new tab you review 2-3-5 cards before doing something else, mixing these tiny chunks of flashcards reviewing through the day, which should also improve memory-retention as interleaving comes into play.RESULTS Upon the conclusion of the test, it was determined that variances of temperature, pH, substrate and enzyme concentration did affect the rate of he reaction.By continuing well assume youre on board with our cookie policy.
While the two are joined, the substrate is converted to its product by catalytic action of the enzyme. There is an active site of the enzyme molecule which is a restricted region that actually attaches to the substrate. Usually the active site is formed by only a few of the enzymes amino acids, the rest is just the framework that reinforces the active site. ![]() Next the substrate is changed to its product, the product is released and the enzymes active site is ready and waiting for another molecule of substrate. Amylase is an enzyme in human saliva and in other organisms and its substrate is starch. When the active site of amylase binds with the starch, hydrolysis takes place. When the hydrolysis (the breaking of a chemical bond with the insertion of the ions of a water molecule) of starch is complete you are left with a saccharine called maltose. The spot plate was labeled in time intervals each two minutes apart. This will demonstrate how long it takes the amylase to hydrology the starch. If the iodine turned blue, the hydrolysis is incomplete and the test was repeated at two minute intervals. The time that elapsed from the beginning of the the reaction is noted. To test the affect of temperature differences on the reaction 4 test tubes with a starchamylase mixture were labeled at different degrees C. C, ICC, ICC, ICC. The test tubes were immersed in 4 water baths that were at the temperature babbled on the test tubes. The procedure noted above with iodine was followed for each test tube and the results documented. To test the effect of pH on the rate of hydrolysis 4 buffered solutions of pH 1. H levels. The appropriate buffer solution was added to each test tube. Next. 5 ml of amylase was added to each test tube. The test tubes were plugged and inverted to mix the contents. Beginning with the test tube with lowest pH, 10 ml of starch as added to each tube. Again the procedure with the iodine was followed and the results documented. To test the effect substrate has on the rate of hydrolysis 4 test tubes were labeled with the following substrate dilutions: 50, 25, 10 and 5. In the 4 test tubes, the following starch solutions were prepared: Dilution Starch Water Ion-II Ion-II ml ml ml ml 5 Mil ml.
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